However, this approach introduces bias based on object size, shape, and distribution. If you can’t count the total number of objects, the next approach might be to determine the density of a small sample, then multiply by the volume of the entire region. This article will introduce you to stereology as a counting tool for electron microscopy, and provides tips for optimizing your approach to get the best results possible, all while using your time efficiently.
But counting these one-by-one isn’t really feasible. You may find yourself needing to count these neurons or connections, which can easily go into the billions. Unpacking the Daunting Task of Stereology for Electron MicroscopyĮlectron microscopy provides fantastic detail and resolution, with brain electron microscopy allowing visualisation of neurons and their individual synaptic connections.
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